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- 產(chǎn)品名稱： RalB Activation Assay Kit
- 產(chǎn)品貨號(hào)： 81501
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 6800
- 數(shù)量：
庫(kù)存： 5
- 規(guī)格： 96T
- 產(chǎn)品信息
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Product Description
Small GTPases are a super-family of cellular signaling regulators. The Ras-like small G proteins, RalA/B,are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.
Currently there is no direct assay to measure the activation of Ral GTPases.
Bioyears Biosciences RalB Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ral-GTP, but not Ral-GDP, and a RalB specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti-Ral-GTP monoclonal antibodies can also be used to monitor the activation of Ral in cells and in tissues by immunohistochemistry.
Bioyears Biosciences RalB Activation Assay Kit provides a simple and fast method to monitor the activation of RalB. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences RalB Activation Assay Kit bases on the configuration-specific anti-Ral-GTP monoclonal antibody to measure the active Ral-GTP levels, either from cell extracts or from in vitro GTPγS loading Ral activation assays. Briefly, anti-active Ral mouse monoclonal antibody will be incubated with cell lysates containing Ral-GTP. The bound active Ral will then be pulled down by protein A/G agarose. The precipitated active RalB or RalA will be detected by immunoblot analysis using anti- RalB or RalA rabbit polyclonal antibody,respectively.
Kit Components
1. Anti-active Ral, Mouse Monoclonal Antibody (Catalog No. 26913): One vial – 22 μL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Ral-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti-RalB, Rabbit Polyclonal Antibody (Catalog No. 21033): One vial – 100 μL (1 mg/ml) in
PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30302): One vial –100 μl at 10 mM, use 5 μL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 μL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4oC until their expiration dates.
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Note
For Research Use Only.
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