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- 產(chǎn)品名稱： Rab35 Activation Assay Kit
- 產(chǎn)品貨號： 82801
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購： 6800
- 數(shù)量：
庫存： 5
- 規(guī)格： 96T
- 產(chǎn)品信息
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Product Description
The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
Currently there is no direct assay to measure the activation of Rab35 GTPases.
Bioyears Biosciences Rab35 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab35 GTP, but not Rab Rab35 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti- Rab35 GTP monoclonal antibodies can also be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab35 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab35. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Rab35 Activation Assay Kit bases on the configuration-specific anti Rab35 GTP monoclonal antibody to measure the active Rab35 GTP levels, either from cell extracts or from in vitro GTPγS loading Rab35 activation assays. Briefly, anti active Rab35 mouse monoclonal antibody will be incubated with cell lysates containing Rab35-GTP. The bound active Rab35 will then be pulled down by protein A/G agarose. The precipitated active Rab35 will be detected by immunoblot analysis using anti Rab35 rabbit polyclonal antibody.
Kit Components
1. Anti active Rab35, Mouse Monoclonal Antibody (Catalog No. 26922): One vial – 22 μL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab35 -GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750
mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab35, Rabbit Polyclonal Antibody (Catalog No. 21078): One vial – 100 μL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 μl at 10 mM, use 5 μL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 μL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4oC until their expiration dates.
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Note
For Research Use Only.
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