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- 產(chǎn)品名稱： Anti RAS(K117N) Mouse Monoclonal
- 產(chǎn)品貨號(hào)： CST26468
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 4800
- 數(shù)量：
庫(kù)存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購(gòu)
Catalog Number
CST26468
Gene Symbol
H-Ras; K-Ras; N-Ras
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Description
Anti RAS(K117N) Mouse Monoclonal Antibody
Background
The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen
A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes K117N mutant, but not wild-type RAS of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant RAS(K117N) and wild-type proteins. Purified His-tagged RAS(K117N) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(K117N) monoclonal antibody (Cat. #26468).
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Immunofluorescence:
Immunofluorescence of cells expressing KRas proteins with anti-KRAS(K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRAS(K117N) plasmid (right column), then fixed and stained with anti-KRAS(K117N) monoclonal antibody (Cat. #26468).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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