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CST26477

H-Ras; K-Ras; N-Ras

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 Anti RAS(G13V) Mouse Monoclonal Antibody

Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

 A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.

 ELISA, WB, IHC

1 mg/ml

mouse

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Liquid

Monoclonal

Purified from ascites

no

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

 Recognizes G13V mutant, but not wild-type RAS of vertebrates.

Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras(G13S) protein (lane 3), His-tagged Ras(G13C) protein (lane 4), His-tagged Ras(G13R) protein (lane 5), His-tagged Ras(G13A) protein (lane 6) were blotted with Anti RAS(G13V) monoclonal antibody (Cat. #26477).   
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Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid, pCDNA3-GFP Ras(G13C) plasmid, pCDNA3-GFP Ras(G13R) plasmid, pCDNA3-GFP Ras(G13A) plasmid or pCDNA3-GFP Ras(G13D) plasmid, then fixed and stained with Anti RAS(G13V) monoclonal antibody (Cat. #26477).

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Store at -20°C. Avoid freeze / thaw cycles

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For Research Use Only.