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- 產(chǎn)品名稱： Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
- 產(chǎn)品貨號： CST26444
- 貨期： 現(xiàn)貨
- 價格與訂購： 4800
- 數(shù)量：
庫存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購
Catalog Number
CST26444
Gene Symbol
NOTCH1, AOS5, AOVD1,TAN1, hN1
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Description
Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
Background
NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
Immunogen
A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
Applications
ELISA, WB, IF, IHC
Recommended Dilutions
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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Immunofluorescence:
Immunofluorescence of cells expressing NOTCH1 proteins with Anti NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP NOTCH1(WT) plasmid (left column) or pCDNA3-GFP NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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